TABLE 2.

Test results for determining the limits of detection of the in-house-developed nsp8 and N gene RT-LAMP-CRISPR assays and the commercial LightMix E gene RT-PCR assay with TNA extracted from Qnostics SARS-CoV-2 Q control 01^a

Concn (copies/mL)	Result
	Intrarun			Interrun
	Test 1	Test 2	Test 3	Test 1	Test 2	Test 3
nsp8 gene RT-LAMP-CRISPR assay
1,000	+	+	+	+	+	+
750	+	+	+	+	+	+
500	+	+	+	−	−	−
250	+	−	−	−	−	−
N gene RT-LAMP-CRISPR assay
4,000	+	+	+	+	+	+
2,000	+	+	+	+	+	+
1,000	+	+	+	+	+	−
500	−	−	−	+	+	+
LightMix E gene RT-PCR assay
125	+	+	+	+	+	+
62.5	+	+	+	+	+	+
31.3	+	+	+	+	+	+
15.6	+	−	−	+	+	−

^a+, detected; −, not detected.