TABLE 3.

Diagnostic performance of the in-house-developed nsp8 and N gene RT-LAMP-CRISPR assays compared to the LightMix E gene RT-PCR assay for SARS-CoV-2 detection

Molecular assay	Molecular assay result	LightMix E gene RT-PCR		Sensitivity (%)	Kappa value (95% CI)a	P value by McNemar’s test
		Positive	Negative
nsp8 RT-LAMP-CRISPR
Nasopharyngeal specimensb	Positive	78	0	100	1	1
	Negative	0	71
Saliva specimensc	Positive	68	0	97.1	0.976 (0.942–1)	0.5
	Negative	2e	100
All specimensd	Positive	146	0	98.6	0.987 (0.97–1)	0.5
	Negative	2e	171
N RT-LAMP-CRISPR
Nasopharyngeal specimensb	Positive	78	0	100	1	1
	Negative	0	71
Saliva specimensc	Positive	68	0	97.1	0.976 (0.942–1)	0.5
	Negative	2e	100
All specimensd	Positive	146	0	98.6	0.987 (0.97–1)	0.5
	Negative	2e	171

^aCI, confidence interval.

^bMedian Cp, 21.8 (range, 14.2 to 33.5).

^cMedian Cp, 24.7 (range, 14.6 to 36.6).

^dMedian Cp, 23.1 (range, 14.2 to 36.6).

^eThe Cp values were 36.2 and 36.6.