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The guide RNAs and ssDNA reporter used for CRISPR in this studya
| gRNA (reference[s]) or ssDNA reporter | Sequence (5′–3′) |
|---|---|
| SARS-CoV-2_nsp8_gRNA | UAAUUUCUACUAAGUGUAGAUCUUAUGCAUCAGCAUUGUGGGA |
| SARS-CoV-2_N_gRNA (21, 24) | UAAUUUCUACUAAGUGUAGAUCCCCCAGCGCUUCAGCGUUC |
| RNaseP-POP7_gRNA (21) | UAAUUUCUACUAAGUGUAGAUAAUUACUUGGGUGUGACCCU |
| ssDNA reporter | FAM-TTATTATT-IABkFQ |
ssDNA, single-stranded DNA; FAM, 6-carboxyfluorescein. IABkFQ, Iowa Black Fluorescent Quencher.
