(a) Genomic alignment of pCY814036-iucA (GenBank accession no. CP093152) with other iucA-harboring virulence plasmids, including pVir_115011 (accession no. CP089955), pSCH6109-Vir (accession no. NZ_CP050860), p130411-38618_1 (accession no. MK649826), pM1026-3Ar.1 (accession no. NZ_CP063859.1), pRGF99-1-214k (accession no. NZ_CP075553.1), pBSI128_vf_res (accession no. NZ_MT269849.1), and p205880-2FIIK (accession no. NZ_MN824002.1). The circular map of plasmids was generated with BLAST Ring Image Generator (BRIG). Different regions harboring multidrug resistance are observed between plasmids. (b and c) Alignment of virulence (b) and resistance (c) regions in plasmids pCY814036-iucA, pVir_115011, pSCH6109-Vir, and p130411-38618_1 using Easyfig. Yellow and purple represent antibiotic resistance and virulence genes, respectively. Red represents insertion sequences and transposons. Unidentified open reading frames (ORFs) are shown in blue, and gray shading indicates nucleotide identity.