TABLE 2.
Mitochondrial proteins significantly changed in Δfut1s parasitesa
| Cluster pattern | Protein name | TriTrypDB ID | Difference in log2(LFQ intensity) |
Corrected ANOVA P value | ||
|---|---|---|---|---|---|---|
| Δlpg2– vs WT | Δfut1s vs WT | Δfut1s vs Δlpg2– | ||||
| Down in Δfut1s | 3-HMG-CoA reductase) (EC 1.1.1.34) | LmjF.30.3190 | 0.87 | −1.38 | −2.26 | 0.01 |
| Aldehyde dehydrogenase, mitochondrial (EC 1.2.1.3) | LmjF.25.1120 | 0.12 | −2.00 | −2.13 | 0.01 | |
| Chaperonin HSP60, mitochondrial | LmjF.36.2030 | 0.80 | −0.81 | −1.61 | 0.04 | |
| Kinetoplast-associated protein-like protein | LmjF.27.0240 | 1.60 | −3.80 | −5.40 | 0.00 | |
| Metallopeptidase, clan ME, family M16 (EC 1.10.2.2) | LmjF.35.1380 | 0.26 | −1.14 | −1.40 | 0.04 | |
| Proline dehydrogenase (EC 1.5.5.2) | LmjF.26.1610 | 1.34 | −0.07 | −1.40 | 0.02 | |
| Putative asparaginyl-tRNA synthetase (EC 6.1.1.22) | LmjF.34.2340 | 0.42 | −1.06 | −1.48 | 0.03 | |
| Putative carbamoyl-phosphate synthase (EC 6.3.4.16) | LmjF.16.0590 | 0.56 | −0.69 | −1.25 | 0.05 | |
| Putative cytochrome c oxidase VIII (EC 1.9.3.1) | LmjF.31.1570 | 0.63 | −2.16 | −2.79 | 0.02 | |
| Putative cytochrome c oxidase subunit 10 (EC 1.9.3.1) | LmjF.23.0370 | −1.34 | −2.49 | −1.15 | 0.04 | |
| Putative DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) | LmjF.32.0650 | 0.72 | −0.69 | −1.42 | 0.04 | |
| Putative heat shock protein | LmjF.18.1370 | 0.56 | −0.83 | −1.38 | 0.04 | |
| Putative NADH dehydrogenase (EC 1.6.99.3) | LmjF.36.5380 | 0.47 | −1.20 | −1.67 | 0.04 | |
| Putative translation initiation factor | LmjF.17.1290 | 0.78 | −0.59 | −1.36 | 0.05 | |
| Up in Δfut1s | Inositol phosphosphingolipid phospholipase C like | LmjF.08.0200 | 0.09 | 2.00 | 1.91 | 0.01 |
| Putative 3,2-trans-enoyl-CoA isomerase mitochondrial (EC 1.1.1.35) (EC 5.3.3.8) | LmjF.31.2330 | −0.79 | 2.14 | 2.93 | 0.04 | |
a
Annotated mitochondrial proteins differing significantly in abundance, as determined by ANOVA, between the Δfut1s and WT or Δlpg2– parasites (Fig. 4; Tables S3 and S5). Overall, there were 279 proteins in clusters where proteins decreased in Δfut1s parasites (142 total; 26 mitochondrial, of which 13 are unannotated), increased in Δfut1s parasites (107 total; 17 mitochondrial, of which 15 are unannotated), or decreased in Δfut1s and varied in Δlpg2– parasites (30 total; 13 mitochondrial, of which 12 are unannotated). CoA, coenzyme A.