. 2022 Nov 7;10(6):e03419-22. doi: 10.1128/spectrum.03419-22

TABLE 3.

Relative abundance (based on DNA) and activity (based on RNA) of the 40 predominant OTUs identified on C. pagurus, i.e., the 20 most abundant OTUs in nonaffected areas (NA) and 20 in black spot syndrome affected areas (BS) were considered^a

20 most abundant OTUs in NA	Relative abundance (%) DNA		Wald test	Relative abundance (%) DNA		Wald test
20 most abundant OTUs in NA	NA	BS	Wald test	NA	BS	Wald test
Alphaproteobacteria
OTU_2 (Loktanella)^b	0.82^b	2.95^b	−4.17^b^,^d	1.06^b	5.82^b	−5.43^b^,^e
OTU_7 (Robiginitomaculum)	4.18	2.31	0.80	2.00	1.31	0.64
OTU_16 (Ahrensia)	1.64	0.77	1.13	1.66	0.71	0.67
OTU_15 (Anderseniella)	1.30	1.33	−0.61^c	1.20	1.35	−1.67
OTU_25 (Hyphomonadaceae)	0.93	0.72	0.39	1.07	0.74	1.04
Gammaproteobacteria
OTU_29 (Xanthomonadales)	0.99	0.38	0.66	0.62	0.19	1.42
OTU_24 (Granulosicoccus)	1.16	0.28	1.65	1.49	0.42	1.63
OTU_4^a (Perspicuibacter)	3.41^b	1.09^b	−2.61^b^,^c	3.85^b	1.18^b	−3.90^b^,^d
OTU_13 (Thiotrichaceae)	1.20	1.33	−0.34	0.57	0.88	−0.04^c
OTU_60 (Cellvibrionaceae)	0.83	0	2.51	0.42	0	−0.99
Flavobacteriia
OTU_19 (Tenacibaculum)	2.30	0.19	−0.22	0.87	0.11	−0.18
OTU_10 (Maritimimonas)	2.07	2.46	0.21	0.74	1.15	0.47^c
OTU_37 (Aquibacter)	1.02	0.13	2.00	0.50	0.06	1.49
OTU_52 (Maribacter)	0.93	0.10	2.26	0.20	0.05	1.91
Acidimicrobiia
OTU_14 (Sva0996 marine group)	2.39	0.58	1.79	2.09	0.73	1.26
OTU_21 (Acidimicrobiales)	1.54	0.44	1.53	1.40	0.55	1.33
Nitrospira
OTU_18 (Nitrospira)	1.30	0.38	1.65	1.84	0.36	2.05
Bacilli
OTU_41 (Granulicatella)	1.03	1.10	1.03	0.40	0.82	−0.36
Sphingobacteriia
OTU_38 (Portibacter)	0.86	0.43	1.92	0.51	0.29	1.11
Mollicutes
OTU_6 (Mycoplasma)	4.46	9.20	0.69	3.98	6.52	0
20 most abundant OTUs in BS	Relative abundance (%) DNA		Wald test	Relative abundance (%) DNA		Wald test
20 most abundant OTUs in BS	NA	BS	Wald test	NA	BS	Wald test
Alphaproteobacteria
OTU_2 (Loktanella)^b	0.82^b	2.95^b	−4.16^b^,^d	1.06^b	5.83^b	−5.43^b^,^e
OTU_8 (Pelagibius)	0.02	2.22	−4.69^c	0.04	2.25	−5.28
OTU_7 (Robiginitomaculum)	4.18	2.31	0.80	2	1.31	0.64
OTU_17 (Sulfitobacter)	0.32	0.93	−1.94	0.44	0.85	−2.27
OTU_15 (Anderseniella)	1.30	0.78	−0.61	1.20	1.35	−1.67
OTU_16 (Ahrensia)	1.64	0.77	1.13	1.66	0.71	0.67
OTU_23 (Thalassobius)	0.62	0.96	−0.99	0.62	0.95	−0.43
Gammaproteobacteria
OTU_3 (Perspicuibacter)	0.05	4.98	−3.13^f	0.02	4.78	−4.07
OTU_4 (Perspicuibacter)^b	3.41^b	1.09^b	−2.61^b^,^c	3.85^b	1.18^b	−3.90^b^,^d
OTU_5 (Eionea)	0	1.67	−0.90	0	1.09	−6.08
OTU_11 (Cocleimonas)	0.76	1.32	−0.74	0.37	0.89	0.04
OTU_12 (Arenicella)^b	0.23^b	1.38^b	−4.17^b^,^d	0.20^b	1.01^b	−4.87^b^,^e
OTU_13 (Thiotrichaceae)	1.20	1.33	−0.34	0.57	0.88	−0.04
OTU_9 (Cellvibrionaceae)^b	0.01^b	2.16^b	−6.43^b^,^e	0^a	2.12^b	−11.47^b^,^e
Flavobacteriia
OTU_1 (Aquimarina)^b	0.31^b	18.67^b	−6.86^b^,^e	0.18^b	14.51^b	−7.29^b^,^e
OTU_10 (Maritimimonas)	2.07	2.46	0.21	0.74	1.15	0.47
Mollicutes
OTU_6 (Mycoplasma)	4.46	9.20	0.69	3.98	6.52	0
Bacilli
OTU_41 (Granulicatella)	1.03	1.10	1.03^b	0.40	0.82	−0.36
Betaproteobacteria
OTU_68 (Comamonadaceae)	0.22	1.10	−1.17	0.26	1.17	−0.78
Deltaproteobacteria
OTU_20 (Eel-36e1D6)	0.03	0.75	−1.55	0.03	2.84	−2.71

Classification of OTUs is given on class level and lowest definable taxonomic level (in brackets).

Significant changes in abundance based on Wald statistics.

Significance for differences in abundance based on Wald statistic was not calculated due to violation of negative binomial distribution or low counts.

P < 0.01, with the significance level after false discovery rate adjustment following Benjamini and Hochberg (53).

P < 0.001, with the significance level after false discovery rate adjustment following Benjamini and Hochberg (53).

P < 0.05, with the significance level after false discovery rate adjustment following Benjamini and Hochberg (53).