TABLE 4.
Taxonomic affiliation based on 16S rRNA gene sequences of new bacterial strains isolated from the carapace of Cancer pagurus specimens. Given are our source (NA = nonaffected or BS = black spot shell diseased areas), the closest described relative (accession number), the similarity of the respective 16S rRNA gene sequence, and the medium used for isolation
| Class/family | Strain | Source | Closest described relativea | 16S rRNA similarity (%) | Mediumb |
|---|---|---|---|---|---|
| Alphaproteobacteria | |||||
| Rhodobacteraceae | |||||
| CP132 | BS | Aliiroseovarius halocynthiae MA1-10 (NR_109323) | 98.9% | 1/2 MB | |
| CP162 | BS | Loktanella acticola OISW-6 (NR_158144) | 98.1% | 1/2 MB | |
| CP110 | BS | Loktanella acticola OISW-6 (NR_158144) | 98.1% | 1/2 MB | |
| CP17 | BS | Loktanella ponticola W-SW2 (NR_134070) | 99.2% | 1/2 MB | |
| CP135 | BS | Octadecabacter ascidiaceicola RA1-3 (NR_147751) | 99.5% | 1/2 MB | |
| CP157 | BS | Paracoccus carotinifaciens E-396 (NR_024658) | 99.7% | 1/2 MB | |
| CP35 | NA | Paracoccus marcusii MH1 (NR_044922) | 98.6% | 1/2 MB | |
| CP32 | NA | Paracoccus marcusii MH1 (NR_044922) | 100% | 1/2 MB | |
| CP137 | NA | Paracoccus yeei G1212 (NR_029038) | 100% | 1/2 MB | |
| CP152 | BS | Roseobacter litoralis Och 149 (NR_074143) | 98.8% | 1/2 MB | |
| CP127 | NA | Ruegeria faecimaris HD-28 (NR_104546) | 100% | 1/2 MB | |
| CP196 | NA | Ruegeria faecimaris HD-28 (NR_104546) | 98.8% | 1/2 MB | |
| CP188 | BS | Ruegeria meonggei MA-E2-3 (NR_118639) | 100% | 1/2 MB | |
| CP187 | BS | Ruegeria meonggei MA-E2-3 (NR_118639) | 100% | 1/2 MB | |
| CP176 | NA | Sulfitobacter litoralis Iso 3 (NR_043547) | 97.0% | 1/2 MB | |
| CP148 | BS | Sulfitobacter marinus SW-265 (NR_043936) | 97.8% | 1/2 MB | |
| Sphingomonadaceae | CP58 | BS | Altererythrobacter confluentis KEM-4 (NR_152077) | 94.8% | 1/2 MB |
| CP16 | BS | Sphingomonas panni C52 (NR_042193) | 99.1% | 1/2 MB | |
| Gammaproteobacteria | |||||
| Colwelliaceae | CP74 | BS | Colwellia aestuarii SMK-10 (NR_043509) | 98.3% | 1/2 MB |
| CP119 | NA | Colwellia polaris 539-7 (NR_043462) | 98.7% | 1/2 MB | |
| Moraxellaceae | CP102 | BS | Acinetobacter lwoffii JCM 6840 (NR_113346) | 100% | 1/2 MB |
| CP138 | NA | Psychrobacter nivimaris 88/2-7 (NR_028948) | 99.7% | 1/2 MB | |
| CP63 | NA | Psychrobacter nivimaris 88/2-7 (NR_028948) | 99.9% | 1/2 MB | |
| CP105 | BS | Psychrobacter nivimaris 88/2-7 (NR_028948) | 99.5% | 1/2 MB | |
| Pseudoalteromonadaceae | |||||
| CP103 | BS | Pseudoalteromonas aliena KMM 3562 (NR_025775) | 100% | 1/2 MB | |
| CP124 | BS | Pseudoalteromonas tetraodonis GFC (CP011041) | 99.3% | 1/2 MB | |
| Shewanellaceae | CP28 | BS | Shewanella marinintestina IK-1 (NR_024791) | 99.7% | 1/2 MB |
| CP56 | BS | Shewanella piezotolerans WP3 (NR_074738) | 98.3% | 1/2 MB | |
| Thiotrichaceae | CP190 | NA | Cocleimonas flava KMM 3898 (NR_112909) | 100% | 1/2 MB |
| CP200 | NA | Cocleimonas flava KMM 3898 (NR_112909) | 99.4% | 1/2 MB | |
| CP122 | BS | Leucothrix mucor ATCC 25107 (NR_118099) | 98.1% | 1/2 MB | |
| CP123 | NA | Leucothrix mucor ATCC 25107 (NR_118099) | 99.7% | 1/2 MB | |
| CP160 | NA | Leucothrix mucor ATCC 25107 (NR_118099) | 99.7% | 1/2 MB | |
| Vibrionaceae | CP69 | BS | Enterovibrio norvegicus LMG 19839 (NR_042082) | 99.3% | 1/2 MB |
| CP129 | BS | Vibrio atlanticus VB 11.11 (NR_116067) | 99.1% | P-SWM | |
| CP45 | NA | Vibrio atlanticus VB 11.11 (NR_116067) | 99.5% | CP-SWM | |
| CP29 | BS | Vibrio crassostreae LGP 7 (NR_114909) | 99.3% | CN-SWM | |
| CP92 | NA | Vibrio crassostreae LGP 7 (NR_044078) | 99.3% | 1/2 MB | |
| CP50 | BS | Vibrio chagasii LMG 21353 (NR_117891) | 99.5% | 1/2 MB | |
| CP41 | NA | Vibrio cyclitrophicus LMG21359 (NR_115806) | 99.9% | CP-SWM | |
| CP46 | NA | Vibrio gigantis LGP 13 (NR_114910) | 99.6% | AC-SWM | |
| CP62 | BS | Vibrio gigantis LGP 13 (NR_044079) | 99.4% | CN-SWM | |
| CP139 | NA | Vibrio gigantis LGP 13 (NR_114910) | 100% | CN-SWM | |
| CP193 | BS | Vibrio splendidus ATCC 33125 (NR_119060) | 99.2% | CP-SWM | |
| CP134 | NA | Vibrio lentus 4OM4T (NR_028926) | 98.5% | CN-SWM | |
| CP68 | BS | Vibrio pectenicida LMG 19642 (NR_118241) | 99.7% | AC-SWM | |
| Flavobacteriia | |||||
| Flavobacteriaceae | CP19 | NA | Aquimarina algiphila 9Alg 151 (NR_159894) | 98.1% | 1/2 MB |
| CP11 | BS | Aquimarina macrocephali JAMB N27 (NR_112971) | 100% | 1/2 MB | |
| CP23 | NA | Aquimarina macrocephali JAMB N27 (NR_112971) | 100% | 1/2 MB | |
| CP22 | NA | Aquimarina macrocephali JAMB N27 (NR_112971) | 100% | 1/2 MB | |
| CP8 | BS | Aquimarina muelleri KMM 6020 (NR_025823) | 98.8% | 1/2 MB | |
| CP111 | BS | Aquimarina muelleri KMM 6020 (NR_025823) | 98.5% | 1/2 MB | |
| CP113 | BS | Aquimarina muelleri KMM 6020 (NR_025823) | 98.8% | 1/2 MB | |
| CP51 | BS | Aquimarina muelleri KMM 6020 (NR_025823) | 98.4% | 1/2 MB | |
| CP43 | NA | Lacinutrix jangbogonensis PAMC 27137 (NR_134754) | 98.0% | 1/2 MB | |
| CP1 | NA | Lacinutrix jangbogonensis PAMC 27137 (NR_134754) | 98.0% | 1/2 MB | |
| CP57 | NA | Maribacter dokdonensis DSW-8 (NR_043294) | 99.7% | 1/2 MB | |
| CP12 | NA | Maribacter forsetii KT02ds18-6 (NR_042627) | 99.7% | 1/2 MB | |
| CP194 | BS | Maribacter stanieri KMM 6046 (NR_116052) | 98.7% | 1/2 MB | |
| CP14 | BS | Tenacibaculum gallaicum A37.1 (NR_042631) | 99.5% | 1/2 MB | |
| CP52 | BS | Tenacibaculum gallaicum A37.1 (NR_042631) | 99.6% | 1/2 MB | |
| CP118 | NA | Winogradskyella litoriviva KMM 6491 (NR_137338) | 97.3% | 1/2 MB | |
| CP175 | NA | Zobellia russellii KMM 3677 (NR_024828) | 99.9% | 1/2 MB | |
| Actinobacteria | |||||
| Nocardioidaceae | CP18 | NA | Nocardioides cavernae YIM A1136 (NR_156135) | 99.2% | 1/2 MB |
| Micrococcaceae | CP30 | NA | Arthrobacter agilis DSM 20550 (MN080900) | 98.3% | P-SWM |
| Bacilli | |||||
| Bacillaceae | CP2 | NA | Bacillus aquimaris TF-12 (NR_025241) | 99.1% | P-SWM |
| CP360 | BS | Bacillus cereus ATCC 14579 (MN326684) | 97.4% | CP-SWM | |
| CP78 | BS | Bacillus frigoritolerans DSM 8801 (MK424281) | 100% | 1/2 MB | |
| CP112 | NA | Bacillus simplex NBRC 15720 (CP017704) | 100% | P-SWM | |
| CP5 | BS | Bacillus aquimaris TF-12 (NR_025241) | 99.0% | 1/2 MB | |
| CP70 | BS | Psychrobacillus psychrodurans 68E3 (NR_025409) | 96.1% | 1/2 MB | |
| Aerococcaceae | CP97 | NA | Aerococcus urinaeequi CCUG28094 (CP014162) | 99.9% | 1/2 MB |
| Staphylococcaceae | CP100 | NA | Staphylococcus equorum PA 231 (NR_027520) | 100% | P-SWM |
| CP24 | BS | Staphylococcus equorum PA 231 (NR_027520) | 100% | 1/2 MB | |
| CP141 | NA | Staphylococcus saprophyticus ATCC 15305 (NR_074999) | 100% | 1/2 MB |
a
Affiliation identified by BLAST analysis (http://blast.ncbi.nlm.nih.gov/Blast.cgi).
b
Abbreviations for used mediums: 1/2 MB, Marine Broth 2216 with 50% peptone and yeast extract; P-SWM, salt water medium44 supplemented with 0.1% (w/v) peptone; CP-SWM, SWM supplemented with 0.2% (w/v) purified chitin and 0.01% (w/v) peptone; AC-SWM, SWM supplemented with 0.2 autoclaved pisteled carapace material from Cancer pagurus; CN-SWM, SWM supplemented with 0.1% (w/v) purified chitin and 0.01 (w/v) N-acetylglucosamine.