TABLE 2.

LOH in CLIB214-derived strains modified using CRISPR-Cas9 technology^e

Strain no.	Strain	Cas9 cut site	LOH coordinatesa	LOH range (kb)a
1	e101740	Chr1: 386,438	Chr5: 104,538 to 661,431	557
2	e205070	Chr2: 1,028,668	Chr2: 1,022,177 to 1,024,638	6.5
3	e301780	Chr4: 417,148	None
4	e301940	Chr3: 898,895	Chr2: 1,540,134 to 1,619,639	79
5	e802210	Chr8: 504,595	Chr8: 142,590 to 567,069	362b,c,d
6	e804640	Chr8: 1,033,704	Chr8: 142,590 to 1,041,458	899b,d
7	e804830	Chr8: 1,080,807	Chr8: 142,590 to 1,078,006	938b
8	e804940	Chr8: 1,097,623	None
9	e805700	Chr8: 1,290,224	Chr8: 1,292,562 to 1,446,962	154
10	805700Δ/Δ	Chr8: 1,288,808	Chr8: 142,590 to 1,296,622	1,146b,c
11	e806320	Chr8: 1,436,180	None
12	“del”805700	Chr8: 1,290,224	Chr8: 1,292,562 to 1,446,962	154

^aLOH coordinates identify the positions of the first and last variants in the LOH block. The LOH range is defined as the distance from the cut site to the most distant homozygous site. If LOH is observed on nontargeted chromosomes, the LOH range is defined as the distance between the first and the last variant in the LOH block.

^bLikely reaches the telomere.

^cThe LOH also extends on the other side of the cut site in e802210 and 805700Δ/Δ (62 kb and 8 kb, respectively). See Fig. 2B.

^dIn e802210 and e804640, there are two and one heterozygous variants, respectively, near the end of the LOH tract (see Table S1 in the supplemental material); this may be due to two adjacent regions of LOH or an error in basecalling.

^eGray color highlights LOH starting at or near the Cas9 cut site. All the strains contain a premature stop codon, except 805700Δ/Δ, in which the target gene was deleted.