TABLE 3.
Oligonucleotide probes targeting 16S rRNA used in this study
| Probe | Fluorophorea | Sequence (5′–3′) | Competitor sequenceb (5′–3′) | FAc (%) | Target | Source |
|---|---|---|---|---|---|---|
| S-D-Arch-0915-a-A-20 | Alexa Fluor 448 | GTGCTCCCCCGCCAATTCCT | 35 | Archaea | 74, 77 | |
| Nm_OL_703 | Cy5 | GCCATCGATGTTCTTCCATATCTC | GCCATCGGTGTTCCTCCATATCTC GCCATCGGTGTTCCTCCACATCTC |
35 |
N. oligotropha, N. ureae, N. aestuarii, N. marina lineage (cluster 6a + 6b) |
75, 78 |
a
Probes were labeled at the 5’ and 3’ ends.
b
Competitors were unlabeled oligonucleotides and added at the same molar concentrations as the labeled probes.
c
Formamide, with the concentration in the hybridization buffer (vol/vol) indicated in parentheses.