TABLE 1.
Methodological comparison of the AS-PCR-LFA platform and nested PCR with sequencing in different levels of parasitemia
| Genotype and parasitemia (parasites/μL) | Method |
Sensitivity (%) | Specificity (%) | False negative (%) | False positive (%) | |||
|---|---|---|---|---|---|---|---|---|
| Sequencing, no. | AS-PCR-LFAa |
|||||||
| WT | Mut | Mix | ||||||
| A256T | ||||||||
| Low (≤1,000) | 53 | 44 | 6 | 2 | 98.11 | 100 | 1.89 | 0 |
| Medium (1,001–9,999) | 49 | 42 | 6 | 0 | 97.96 | 100 | 2.04 | 0 |
| High (≥10,000) | 175 | 145 | 28 | 1 | 99.43 | 100 | 0.57 | 0 |
| Subtotal | 277 | 231 | 40 | 3 | 98.92 | 100 | 0.36 | 0 |
| A551T | ||||||||
| Low (≤1,000) | 53 | 15 | 18 | 15 | 90.57 | 100 | 9.43 | 0 |
| Medium (1,001–9,999) | 49 | 17 | 27 | 4 | 97.96 | 100 | 2.04 | 0 |
| High (≥10,000) | 175 | 66 | 90 | 13 | 96.57 | 100 | 2.86 | 0 |
| Subtotal | 277 | 98 | 135 | 32 | 95.67 | 100 | 4.33 | 0 |
a
WT, Mut, and Mix represent the wild type, mutation, and mixed type, respectively.