. 2022 Oct 31;10(6):e01966-22. doi: 10.1128/spectrum.01966-22

TABLE 2.

Detection of microorganism in healthy, cervicitis, and vaginitis groups based on the cutoff values of the NAR

Microorganism	Healthy controls vs cervicitis patients					Healthy controls vs vaginitis patients
	Cutoff (NAR)^a	No. (%) of:		OR (95% CI)^b	P ^c	Cutoff (NAR)^a	No. (%) of:		OR (95% CI)^b	P ^c
	Cutoff (NAR)^a	Healthy controls	Cervicitis patients	OR (95% CI)^b	P ^c	Cutoff (NAR)^a	Healthy controls	Vaginitis patients	OR (95% CI)^b	P ^c
Lactobacillus spp.	>0.84	250 (43.3)	31 (19.5)	0.32 (0.21–0.49)	<0.0001	>0.79	332 (57.4)	72 (34.8)	0.40 (0.28–0.55)	<0.0001
	≤0.84	326 (56.4)	118 (74.2)	2.22 (1.50–3.29)	0.0001	≤0.79	244 (42.2)	127 (61.4)	2.17 (1.57–3.01)	<0.0001
T. vaginalis	>0.39	3 (0.5)	5 (3.1)	6.22 (1.47–26.33)	0.014	>0.41	2 (0.3)	4 (1.9)	5.68 (1.03–31.22)	0.045
	≤0.39	3 (0.5)	1 (0.6)	1.21 (0.13–11.74)	0.868	≤0.41	4 (0.7)	0 (0)		0.430
M. genitalium	>0.46	3 (0.5)	4 (2.5)	4.95 (1.10–22.33)	0.042	>0.47	2 (0.3)	4 (1.9)	5.67 (1.03–31.22)	0.045
	≤0.46	9 (1.6)	0 (0)		0.250	≤0.47	10 (1.7)	0 (0)		0.160
U. urealyticum	>0.55	14 (2.4)	10 (6.3)	2.70 (1.18–6.21)	0.022	>0.55	14 (2.4)	16 (7.7)	3.37 (1.62–7.04)	0.001
	≤0.55	27 (4.7)	3 (1.9)	0.39 (0.12–1.31)	0.129	≤0.55	27 (4.7)	5 (2.4)	0.51 (0.19–1.33)	0.167
T. pallidum	>0.57	0 (0)	6 (3.8)		<0.0001	>0.45	1 (0.2)	14 (6.8)	41.85 (5.47–320.39)	<0.0001
	≤0.57	3 (0.5)	6 (3.8)	7.52 (1.86–30.40)	0.005	≤0.45	2 (0.3)	3 (1.4)	4.24 (0.70–25.53)	0.115
N. gonorrhoeae	>0.40	0 (0)	4 (2.5)		0.002	>0.40	0 (0)	5 (2.4)		0.001
	≤0.40	2 (0.3)	5 (3.1)	9.35 (1.80–48.66)	0.008	≤0.40	2 (0.3)	2 (1.0)	2.81 (0.39–20.08)	0.303
C. trachomatis	>0.65	2 (0.3)	6 (3.8)	11.29 (2.26–56.51)	0.002	>0.23	28 (4.8)	47 (22.7)	5.77 (3.50–9.51)	<0.0001
	≤0.65	40 (6.9)	26 (16.4)	2.63 (1.55–4.46)	0.0003	≤0.23	14 (2.4)	14 (6.8)	2.92 (1.37–6.24)	0.006
K. granulomatis	>0.45	7 (1.2)	6 (3.8)	3.20 (1.06–9.66)	0.041	>0.45	6 (1.0)	9 (4.3)	4.33 (1.52–12.33)	0.006
	≤0.45	40 (6.9)	16 (10.1)	1.50 (0.82–2.77)	0.188	≤0.45	41 (7.1)	21 (10.1)	1.48 (0.85–2.57)	0.165
M. hominis	>0.41	5 (0.9)	10 (6.3)	7.69 (2.59–22.84)	<0.0001	>0.41	5 (0.9)	19 (9.2)	11.58 (4.27–31.45)	<0.0001
	≤0.41	45 (7.8)	8 (5.0)	0.63 (0.29–1.36)	0.238	≤0.41	45 (7.8)	21 (10.1)	1.34 (0.78–2.30)	0.295
U. parvum	>0.47	77 (13.3)	37 (23.3)	1.97 (1.27–3.06)	0.002	>0.30	207 (35.8)	91 (44.0)	1.41 (1.02–1.94)	0.038
	≤0.47	158 (27.3)	40 (25.2)	0.89 (0.60–1.34)	0.583	≤0.30	28 (4.8)	13 (6.3)	1.32 (0.67–2.59)	0.427
H. ducreyi	>0.41	0 (0)	2 (1.3)		0.046	>0.41	0 (0)	2 (1.0)		0.069
	≤0.41	1 (0.2)	0 (0)		0.909	≤0.41	1 (0.2)	0 (0)		0.963
HCMV	>0.40	70 (12.1)	27 (17.0)	1.48 (0.92–2.41)	0.108	>0.38	81 (14.0)	54 (26.1)	2.17 (1.47–3.20)	<0.0001
	≤0.40	53 (9.2)	7 (4.4)	0.46 (0.20–1.02)	0.057	≤0.38	42 (7.3)	6 (2.9)	0.38 (0.16–0.91)	0.030
HHV-8	>0.47	22 (3.8)	10 (6.3)	1.70 (0.79–3.66)	0.174	>0.43	32 (5.5)	25 (12.1)	2.34 (1.35–4.06)	0.002
	≤0.47	38 (6.6)	6 (3.8)	0.56 (0.23–1.34)	0.193	≤0.43	28 (4.8)	9 (4.3)	0.89 (0.41–1.93)	0.773
VZV	>0.38	0 (0)	6 (3.8)		<0.0001	>0.38	0 (0)	8 (3.9)		<0.0001
	≤0.38	11 (1.9)	0 (0)		0.197	≤0.38	11 (1.9)	0 (0)		0.141
HSV-1	>0.33	6 (1.0)	2 (1.3)	1.21 (0.24–6.08)	0.685	>0.21	12 (2.1)	1 (0.5)	0.23 (0.03–1.77)	0.202
	≤0.33	6 (1.0)	0 (0)		0.381	≤0.21	0 (0)	1 (0.5)		0.963
HSV-2	>0.32	2 (0.3)	0 (0)		1.000	>0.32	2 (0.3)	1 (0.5)	1.40 (0.13–15.50)	1.000
	≤0.32	1 (0.2)	0 (0)		0.909	≤0.32	1 (0.2)	0 (0)		0.963

For each microorganism, cutoff values were set according to the Youden index using MedCalc. The cutoff was categorized using the NAR of microbial load (log₁₀ copies/mL)/human RNase P load (log₁₀ copies/mL). The cutoff is the predicted probability of the logistic regression model, where 0 = healthy and 1 = cervicitis or vaginitis.

Odds ratios and 95% CIs were calculated by univariable logistic regression analysis.

Chi-square test and Fisher’s exact test were used to compare variables between groups. Bold indicates significance (P < 0.05).