TABLE 2.
Non structural protein (nsp12) nucleotide substitutions detected in clinical isolatesa
| Mutationb | Location | No. of participants with mutation (days of treatment)c |
No. of participants with: |
|||
|---|---|---|---|---|---|---|
| Pre-RDV | Pre-/post-RDV | Post-RDV | ICU admission | Mortalityd | ||
| A13535G (Y32C) | 2 | 1 (10) | 1 (5) | 1 | ||
| C13551T | 1 (18) | 1 | 1 | |||
| G13564T (V42L) | 1 (5) | |||||
| A13689T (E83D) | 1 (18) | 1 | 1 | |||
| A13711G (K91G) | 1 (5) | |||||
| C14119T | Nsp8 interaction | 1 (5) | ||||
| C14120T (P227L) | Nsp8 interaction | 3 (5 [n = 2], 10 [n = 1]) | 1 (5) | 3 | 1 | |
| C14178T | Nsp8 interaction | 1 (5) | ||||
| C14547A | Nsp7-8 interaction | 2 (5) | 3 (5) | 3 | ||
| C14703T (T422I) | RNA binding site (motif G) | 1 (10) | 1 | |||
| C15237T | RNA binding site (motif B) | 1 (10) | ||||
| C15240T | RNA binding site (motif B) | 1 (5) | 1 | 1 | ||
| C15324T | RNA binding site | 2 | 1 (30) | 2 (5) | 3 | 1 |
| C15441T | RNA binding site (motif C) | 1 (5) | ||||
| G15627T (E729D) | RNA binding site (motif E) | 1 (5) | 1 | |||
| C15652T (D738Y) | RNA binding site | 1 (5) | 1 (5) | 1 | ||
| G15910T | 1 (10) | |||||
| C15952A (L838I) | 8 (10 [n = 7]; 21 [n = 1]) | 1 (20) | 6 | 3 | ||
a
Nsp, non structural protein; RDV, remdesivir; ICU, intensive care unit.
b
The nucleotide position is indicated with respect to the Wuhan-Hu-1 reference genome (GenBank accession number MN908947.3). The amino acid substitution is indicated in parentheses.
c
Number of subjects with the specific substitution at baseline (pre-RDV), at baseline and after treatment with remdesivir (pre-/post-RDV), and after (post-RDV) therapy. The number of days of treatment received when the mutation was found is indicated in parentheses.
d
Number of participants who died due to all-cause mortality.