TABLE 2.
Fusarium species overall performance of databasesg
| FSSCa (n = 6) |
FFSCa (n = 6) |
FOSCa (n = 3) |
FIESCa (n = 3) |
All Fusarium spp. (n = 18) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Level of ID |
BT n (%) |
MSI-2 n (%) |
BT n (%) |
MSI-2 n (%) |
BT n (%) |
MSI-2 n (%) |
BT n (%) |
MSI-2 n (%) |
BT n (%) |
MSI-2 n (%) |
| Species | 1 (16.7%) | 2 (33.3%) | 6 (100%) | 5 (83.3%) | 0 (0%) | 0 (0%) | 0b (0%) | 0b (0%) | 7b (43.8%) | 7b (43.8%) |
| Species Complex | 5 (83.3%)c,d | 6 (100%)c,d | 6 (100%) | 6 (100%) | 3 (100%)c,e | 3 (100%) | 3 (100%)c,f | 3 (100%)c,f | 17 (94.4%) | 18 (100%) |
| Genus | 6 (100%) | 6 (100%) | 6 (100%) | 6 (100%) | 3 (100%) | 3 (100%) | 3 (100%) | 3 (100%) | 18 (100%) | 18 (100%) |
| No ID | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
FSSC, Fusarium solani species complex; FFSC, Fusarium fujikuroi species complex; FOSC, Fusarium oxysporum species complex; FIESC, Fusarium incarnatum-equiseti species complex.
Denominator is smaller for species versus others classification levels.
Misidentification to the classification level above.
2/5 isolates misidentified to species, but correctly identified to species complex.
3/3 isolates misidentified to species, but correctly identified to species complex.
1/3 isolates misidentified to species, but correctly identified to species complex.
Performance of each database (Bruker Filamentous Fungi v3 [BT] versus Mass Spectral Identification-2 [MSI-2]) by species (n = 16), species complex (n = 18), and genus (n = 18). The best score was taken from each replicate. The reference standard was translation elongation factor sequencing and DNA phylogenetic tree-based approach analysis.