FIG 1.

DEV US3 inhibits IFN-β production induced by ISD and poly(dA-dT). (A and B) DEFs were transfected with US3 expression plasmid or empty vector for 24 h and then transfected with ISD (1 μg/mL); the mRNA levels of IFN-β, IL-6, Mx, and OASL were measured by qPCR 6 h and 12 h later. (C and D) DEFs were transfected with US3 expression plasmid or empty vector for 24 h and then transfected with poly(dA-dT) (1 μg/mL); the mRNA levels of IFN-β, IL-6, Mx, and OASL were measured by qPCR 6 h and 12 h later. The relative amounts of IFN-β, IL-6, Mx, and OASL mRNA were normalized to β-actin mRNA levels in each sample, and the fold differences between the treated samples and the mock controls were calculated. All controls and treated groups were tested in triplicate. *, P < 0.05; **, P < 0.01.