FIG 4.

DEV US3 phosphorylates IRF7 and blocks IRF7 dimerization and nuclear translocation. (A) IRF7-HA was cotransfected with empty vector or US3- or US3K213A-expressing plasmid into DEFs. The cells were lysed 36 h later and left untreated or treated with lambda PP prior to SDS-PAGE and Western blotting. (B) DEFs were transfected with empty vector or US3- or US3K213A-expressing plasmid. The cells were lysed 36 h later, and Western blotting was performed with anti-IRF7 antibodies to detect IRF7 phosphorylation. (C) DEFs were transfected with the indicated plasmids for 36 h before coimmunoprecipitation and immunoblot analysis with the indicated antibodies. (D) DEFs were transfected with US3 or US3K213A plasmid; 24 h later, cells were either left untreated or transfected with ISD for 12 h before confocal microscopy. Bars, 10 μm.