TABLE 3.
Characterization of M. abscessus MMV688845-resistant mutants
| M. abscessus subsp. abscessus strain | Batch | MIC90 (μM)a of MMV688845 | RpoB mutation(s) |
|---|---|---|---|
| Bamboo | 8 | ||
| Bamboo 845R-1.1 | 1 | >100 | P473L |
| Bamboo 845R-2.1 | 2 | >100 | P473L |
| Bamboo 845R-2.2 | 2 | >100 | G562S |
| Bamboo 845R-2.3 | 2 | >100 | L556P, V557P |
| Bamboo 845R-2.4 | 2 | >100 | Q581R |
| Bamboo 845R-2.5 | 2 | >100 | D576Y |
a
Determined by measurement of OD600 and evaluated via method B. Two batches of spontaneous resistant mutants were generated by selection at 50 μM MMV688845. The observed frequency of resistance was 6 × 10−8 CFU−1.