TABLE 1.

Overview of mutations identified in the odhI gene^a

Mutant	Mutation in odhI	Position in odhI ORFb	Codon exchange	Effect on amino acid sequence
K1	GTTTCACGTCGCCAC	260	CGT → CCT	R87P
K2	GGCGCTCGATTCCTT	178	CGA → TGA	R60Stop
K3	GAGATCCAGATTGGC	385	CAG → TAG	Q129Stop
K4	GTTTCACGTCGCCAC	260	CGT → CCT	R87P
K5	CTGGACCAGCCAACC	195	CAG → TAG	Q53Stop
K6	ATGGAGTCGAGCACC	84	TCG → TCA	Silent (S28)
	CACGCAGAGTTCCGC	271	GAG → AAG	E91K
	GTCATGCAGACCGGT	369	CAG → CAA	Silent (Q123)
	GATGAGATCCAGATT	381	GAG → GAA	Silent (E127)
		387	CAG → CAA	Silent (Q129)
K7	GTTTCACGTCGCCAC	260	CGT → CCT	R87P
	CACGCAGAGTTCCGC	271	GAG → AAG	E91K
K8	GGGTCCCTCAACGGA	319	CTC → GTC	L107V
K9	CTGGTTTTCCTCGCA	410	TTC → TCC	F137S
K10	GATGTCACCGTTTCA	251	ACC → ATC	T84I
K11	GCAGGCGCTCGATTC	176	GCT → GCAT	Frameshift after A59
K12	AACGCTCAGGTCATG		CAG → C-G	Frameshift after A119
K13	No mutation in odhI
K14	No mutation in odhI
K15	No mutation in odhI
K16	CACGCAGAGTTCCGC	271	GAG → AAG	E91K
K17	ATGGAGTCGAGCACC	84	TCG → TCC	Silent (S28)
	GCAGGCGCTCGATTC	176	GCT → GAT	A59D
	CTGGACCAGCCAACC	193	CAG → TAG	Q65Stop
K18	No mutation in odhI
K19	AACGCTCAGGTCATG	359	CAG → C-G	Frameshift after A119
K20	GTCATGCAGACCGGT	367	CAG → TAG	Q123Stop

^a20 C. glutamicum ΔglnX2 suppressor mutants (K1 to K20) forming colonies on glutamine agar plates.

^bORF, open reading frame.