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. 2022 Dec 7;12:990398. doi: 10.3389/fonc.2022.990398

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Copyright © 2022 Obr, Bulatowicz, Chang, Ciliento, Lemenze, Maingrette, Shang, Gallagher, LeRoith and Wood

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Epithelial cell populations are altered with reduced IGF1R. (A) UMAP plot of re-clustering of epithelial cells from Wnt1, DN-Wnt1, and K8iKOR-Wnt1 tumors resulting in 13 clusters. (B) Dot plot of epithelial cell markers. (C) Heat map of top epithelial cell type markers. Top legend: top row=tumor identity: red=Wnt1, green=DN-Wnt1, blue=K8iKOR-Wnt1; Bottom row=epithelial cell cluster. (D) Percent tumor genotype graph for each cell cluster labelled with each cell type defined by markers. (ALV=alveolar cell, LUM=luminal cell, DL=differentiated luminal cell, LP=luminal progenitor, BAS=basal cell, BIP=basal bipotential progenitor). (E) Violin plot for Lgr5 in each annotated cluster and tumor type. (F) Representative contour plots of flow cytometry of the CD24⁺/CD29^lo (luminal) and CD24⁺/CD29^hi (basal) cell populations and CD24⁺/CD29^lo/CD61^- (luminal progenitor) cell population in Wnt1 and K8iKOR-Wnt1 tumors. G-I. Quantification of luminal (G), basal (H), and luminal progenitor (I) populations in Wnt1 and K8iKOR-Wnt1 tumors. Each dot represents an individual tumor. Statistic: Unpaired Student’s t-test.