TABLE 2.

Information for Mycobacterium tuberculosis complex drug resistance genes for tNGS assay^a

Gene	Genome positions	Gene positions	Codons	Avg depth (×)
rpoB	759735 to 763385	−72 to +61	−71 to full CDS	2,509
katG	2153865 to 2156228	−116 to +25	−116 to full CDS	1,940
fabG1	1673257 to 1674268	−183 to +86	−183 to full CDS	2,259
inhA	1674135 to 1675020	−67 to +10	−67 to full CDS	2,881
embB	4246468 to 4249822	−46 to +13	−46 to full CDS	1,231
pncA	2288670 to 2289361	−119 to +12	−119 to full CDS	2,101
gyrA	7263 to 9847	−39 to +30	−39 to full CDS	2,057
gyrB	5239 to 7391	−1 to +125	−1 to full CDS	2,478
rrs	1471830 to 1473462	−16 to +81	NA	2,097
eis	2714105 to 2715534	−201 to +20	−201 to full CDS	1,579
rpsL	781549 to 781983	−11 to +50	−11 to full CDS	2,586
atpE	1461005 to 1461310	−40 to +21	−40 to full CDS	3,132
Rv0678	778881 to 779541	−109 to +55	−109 to full CDS	2,079
pepQ	2859258 to 2860442	−23 to +43	−23 to full CDS	1,844
rrl	1473641 to 1476880	−17 to +86	NA	2,636
rplC	800714 to 801472	−95 to +11	−95 to full CDS	2,793
Rv1979c	2221706 to 2223283	−118 to +14	−118 to full CDS	2,608
ddn	3986732 to 3987332	−112 to +34	−112 to full CDS	1,994
fgd1	490636 to 491818	−147 to +26	−147 to full CDS	2,219
fbiA	3640397 to 3641598	−146 to +61	−146 to full CDS	1,520
fbiB	3641476 to 3642891	−59 to +11	−59 to full CDS	2,142
fbiC	1302803 to 1305527	−128 to +27	−128 to full CDS	1,776

^aPositions of the reference sequences relative to the genome and genes of the M. tuberculosis H37Rv strain are indicated. Gene positions in promoter or 3′ region relative to the +1 or last nucleotide of coding sequences (CDS) are indicated by minus or plus signs, respectively. NA, not applicable for codons (positions outside a CDS or in rRNA gene region).