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. Author manuscript; available in PMC: 2022 Dec 21.
Published in final edited form as: Cell Rep. 2022 Nov 22;41(8):111709. doi: 10.1016/j.celrep.2022.111709

Figure 3. Ablation of Col1α1+ fibroblasts aggravates ICH outcome.

Figure 3.

(A) Representative images of cresyl violet staining in control and FKO brains at days 2, 5, and 7 after ICH. Dotted lines indicate hematoma.

(B) Quantification of injury volume in control and FKO mice at days 2, 5, and 7 after ICH. n = 4–5 mice for day 2;n = 5–7 mice for day 5; and n = 8 mice for day 7. **p = 0.0017 and **p = 0.0022 at days 5 and 7 after ICH by Student’s t test, respectively.

(C) Survival rate for control and FKO mice after ICH. n = 16 and 19 mice for control and FKO mice, respectively.

(D) Representative images of FJC (green) and DAPI (blue) in control and FKO brains at days 2 and 7 after ICH. Dotted lines and arrows indicate hematoma. Scale bars, 100 μm.

(E) Quantification of FJC+ cells in the peri-hematoma region in control and FKO mice. n = 4 mice. *p = 0.0286 by Mann-Whitney U test.

(F) Neurological deficit score in control and FKO mice after ICH. n = 16–19 mice for days 1–5 and n = 8–10 mice for days 6 and 7. *p = 0.0269, **p = 0.0027, ***p = 0.0002 by Student’s t test.

Data were shown as mean ± SD. FJC, fluor jade C. See also Videos S1 and S2.