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. Author manuscript; available in PMC: 2022 Dec 21.
Published in final edited form as: Cell Rep. 2022 Nov 22;41(8):111709. doi: 10.1016/j.celrep.2022.111709

Figure 7. TIMP2 treatment enhances BBB integrity in FKO mice in vivo.

Figure 7.

(A) Representative images of TIMP2 (green) and DAPI (blue) in the peri-hematoma regions of saline- and TIMP2-treated FKO mice at day 7 after ICH. Scale bars, 100 μm.

(B) Quantification of TIMP2 intensity in the peri-hematoma regions of saline- and TIMP2-treated mice at day 7 after ICH. n = 4 mice. *p = 0.0286 by Mann-Whitney U test.

(C) Representative images of cresyl violet staining in saline- and TIMP2-treated FKO mice at day 7 after ICH. Dotted lines indicate injury areas.

(D) Quantification of injury volume in saline- and TIMP2-treated mice at day 7 after ICH. n = 5–6 mice. *p = 0.0491 by Student’s t test.

(E) Representative images of hemoglobin (red) and CD31 (green) in the peri-hematoma regions of saline- and TIMP2-treated mice at day 7 after ICH. Scale bars, 100 μm.

(F) Quantification of hemoglobin intensity in the peri-hematoma regions of saline- and TIMP2-treated mice at day 7 after ICH. n = 4 mice. *p = 0.0286 by Mann-Whitney U test.

(G) Representative images of biotin (green) and CD31 (red) in the peri-hematoma regions of saline- and FKO-treated mice at day 7 after ICH. Scale bars, 100 μm.

(H) Quantification of biotin intensity in the peri-hematoma regions of saline- and TIMP2-treated mice. n = 4 mice. *p = 0.0286 by Mann-Whitney U test.

(I) Representative images of ZO-1 (green) and podocalyxin (red) in the peri-hematoma regions of saline- and TIMP2-treated mice at day 7 after ICH. Scale bars, 50 μm.

(J) Quantification of ZO-1 intensity normalized to podocalyxin-positive area in saline- and TIMP2-treated mice. n = 4 mice. *p = 0.0286 by Mann-Whitney U test.

(K) Representative images of occludin (green) and podocalyxin (red) in the peri-hematoma regions of saline- and TIMP2-treated mice at day 7 after ICH. Scale bars, 50 μm.

(L) Quantification of occludin intensity normalized to podocalyxin-positive area in saline- and TIMP2-treated mice. n = 4 mice. *p = 0.0286 by Mann-Whitney U test.

(M) Representative images of PDGFRβ (green) and podocalyxin (red) in the peri-hematoma regions of saline- and TIMP2-treated mice at day 7 after ICH. Scale bars, 50 μm.

(N) Quantification of PDGFRβ coverage over podocalyxin-positive capillaries in saline- and TIMP2-treated mice. n = 4 mice. Data were shown as mean ± SD.