KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-CD31 | BD Biosciences | Cat#553370; RRID:AB_394816 |
| Goat anti-Podocalyxin | R&D | Cat#AF1556; RRID:AB_354858 |
| Rabbit anti-RALDH2 | Sigma | Cat#HPA010022; RRID:AB_1844723 |
| Mouse anti-NG2 | BD Biosciences | Cat#554275; RRID:AB_395339 |
| Mouse anti-Desmin | Millipore | Cat#IF02L; RRID:AB_2261688 |
| Rabbit anti-Olig2 | Novus | Cat#NBP1-28667; RRID:AB_1914109 |
| Rabbit anti-ZO-1 | Innovative Research | Cat#61-7300; RRID:AB_138452 |
| Rabbit anti-AQP4 | Millipore | Cat#AB3594; RRID:AB_91530 |
| Rabbit anti-Col1 | Millipore | Cat#AB765P; RRID:AB_92259 |
| Rabbit anti-Occludin | Invitrogen | Cat#71-1500; RRID:AB_2533977 |
| Mouse anti-Claudin5 | Invitrogen | Cat#35-2500; RRID:AB_2533200 |
| Rat anti-Meca32 | Novus | Cat#NB100-77668; RRID:AB_2276108 |
| Rabbit anti-Caveolin-1 | Cell Signaling | Cat#3238; RRID:AB_2072166 |
| Rabbit anti-PDGFRβ | Cell Signaling | Cat#3169; RRID:AB_2162497 |
| Goat anti-PDGFRα | R&D | Cat#AF1062; RRID:AB_2236897 |
| Goat anti-TIMP2 | R&D | Cat#AF971; RRID:AB_355752 |
| Mouse anti-PAI1 | R&D | Cat#MAB1786; RRID:AB_2186903 |
| Mouse IgG | R&D | Cat#MAB002; RRID:AB_357344 |
| Rat anti-CD13-FITC | BD Biosciences | Cat#558744; RRID:AB_397101 |
| Rabbit anti-Hemoglobin | Cloud-Clone | Cat#PAB409Mu01 |
| Mouse anti-Actin | Sigma | Cat#A5441; RRID:AB_476744 |
| Alexa Fluor-405 conjugated donkey anti-rat | Invitrogen | Cat#A48268; RRID:AB_2890549 |
| Alexa Fluor-488 conjugated donkey antirabbit | Invitrogen | Cat#A21206; RRID:AB_2535792 |
| Alexa Fluor-594 conjugated donkey antirabbit | Invitrogen | Cat#A21207; RRID:AB_141637 |
| Alexa Fluor-594 conjugated donkey anti-mouse | Invitrogen | Cat#A21203; RRID:AB_141633 |
| Alexa Fluor-594 conjugated donkey anti-rat | Invitrogen | Cat#A21209; RRID:AB_2535795 |
| Alexa Fluor-647 conjugated goat anti-rat | Invitrogen | Cat#A21247; RRID:AB_141778 |
| FITC conjugated goat anti-mouse | BD Pharmingen | Cat#554001; RRID:AB_395197 |
| FITC conjugated goat anti-rat | BD Pharmingen | Cat#554016; RRID:AB_395210 |
| Bacterial and virus strains | ||
| Lentivirus-siRNA-TIMP2 | Santa Cruz | Cat#SC-29506 |
| Lentivirus-siRNA-Control | Santa Cruz | Cat#SC-108080 |
| Chemicals, peptides, and recombinant proteins | ||
| Diphtheria Toxin | Sigma-Aldrich | Cat#D0564 |
| Sulfo-NHS-Biotin | ThermoFisher | Cat#21217 |
| Avidin-FITC | BD Biosciences | Cat#554057 |
| Recombinant Human TIMP-2 Protein | R&D | Cat#971-TM-010 |
| Critical commercial assays | ||
| RNAScope in situ hybridization | Advanced Cell Diagnostics | Cat#323100 |
| Deposited data | ||
| Raw and processed MS data | ProteomeXchange Consortium | Database: PXD037247 |
| Experimental models: Cell lines | ||
| Mouse brain endothelial cells (bEnd.3) | ATCC | Cat#CRL-2299; RRID:CVCL_0170 |
| Mouse bone marrow cells (LADMAC) | ATCC | Cat#CRL-2420; RRID:CVCL_2550 |
| Mouse brain microglia (EOC 13.31) | ATCC | Cat#CRL-2468; RRID:CVCL_5743 |
| Human brain microvascular endothelial cells (HBMEC) | ScienCell | Cat#1000 |
| Human brain vascular adventitial fibroblasts (HBVAF) | ScienCell | Cat#1110 |
| Experimental models: Organisms/strains | ||
| Mouse: B6.FVB-Tg(Col1a1-cre)1Kry | Riken BRC | BRC: RBRC05603; RRID:IMSR_RBRC05603 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | JAX:007914; RRID:IMSR_JAX:007914 |
| Mouse: C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | The Jackson Laboratory | JAX:007900; RRID:IMSR_JAX:007900 |
| Software and algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Photoshop | Adobe | N/A |