Table 2.
Statistics of differentially accumulating secondary metabolite in CK and 35S:PS1 transgenic Arabidopsis
| Class | Compounds | Peak area | P | FC | VIP ≥ 1 | Type | |
|---|---|---|---|---|---|---|---|
| CK | GlPS1 | ||||||
| Coumarins | Aesculetin | 50,838 ± 23,536 | 138,087 ± 7375 | 0.02 | 2.72 | 1.13 | Up |
| Coumarins | Daphnetin | 44,132 ± 21,347 | 139,460 ± 3102 | 0.01 | 3.16 | 1.22 | Up |
| Coumarins | Isoscopoletin | 12,829,667 ± 2,622,924 | 81,176,333 ± 2,510,380 | 0.00 | 6.33 | 1.57 | Up |
| Coumarins | 4-Methylesculetin | 265,270 ± 73,755 | 1,009,583 ± 77,513 | 0.00 | 3.81 | 1.33 | Up |
| Coumarins | 6-Hydroxy-7-methoxy-coumarin | 3,096,100 ± 1,584,212 | 16,908,333 ± 510,308 | 0.00 | 5.46 | 1.50 | Up |
| Coumarins | 8-Methoxypsoralen | 9 ± 0 | 80,331 ± 12,445 | 0.00 | 8.93 | 3.49 | Up |
| Coumarins | Isopimpinellin | 9 ± 0 | 25,894 ± 2008 | 0.00 | 2.88 | 3.27 | Up |
| Coumarins | Imperatorin | 9 ± 0 | 134,537 ± 6147 | 0.00 | 1.49 | 3.59 | Up |
| Coumarins | Isooxypeucedanine | 9 ± 0 | 129,987 ± 5478 | 0.00 | 1.44 | 3.59 | Up |
| Coumarins | Phellopterin | 12,178 ± 4061 | 443,910 ± 24,754 | 0.00 | 3.65 | 2.20 | Up |
| Coumarins | Wedelolactone | 9 ± 0 | 19,161 ± 5170 | 0.02 | 2.13 | 3.20 | Up |
| Coumarins | Esculin | 9 ± 0 | 6,645,067 ± 151,901 | 0.00 | 7.38 | 4.26 | Up |
| Glucosinolates | Gluconapin | 253,103 ± 26,409 | 650,300 ± 70,250 | 0.00 | 2.57 | 1.12 | Up |
| Glucosinolates | Glucosinalbin | 9 ± 0 | 1,512,167 ± 68,381 | 0.00 | 1.68 | 4.02 | Up |
| Alkaloids | Caffeoylcholine 5-glucoside | 9 ± 0 | 74,471 ± 11,383 | 0.00 | 8.27 | 3.48 | Up |
| Alkaloids | Feruloylcholine glucoside | 9 ± 0 | 20,634 ± 1846 | 0.00 | 2.29 | 3.22 | Up |
| Alkaloids | N-isobutyl-4,5-epoxy-2E-decaenamide | 126,642 ± 112,367 | 3,310,333 ± 155,769 | 0.00 | 2.61 | 2.13 | Up |
| Alkaloids | Camalexin | 370,595 ± 298,636 | 23,468,333 ± 962,923 | 0.00 | 6.33 | 2.41 | Up |
| Alkaloids | Cocamidopropyl βine | 2,299,567 ± 1,453,144 | 50,595,333 ± 4,161,829 | 0.00 | 2.20 | 2.05 | Up |
| Flavonols | Isorhamnetin-O-Hexoside-O-rhamnoside-O-rhamnoside | 9 ± 0 | 51,902 ± 12,447 | 0.02 | 5.77 | 3.40 | Up |
| Flavonols | Kaempferol-3-O-rhamnosyl-sophorotrioside-7-O-glucoside | 191,457 ± 63,010 | 43,307 ± 7138 | 0.05 | 2.30 | 1.36 | Down |
| Phenolic acids | Caffeic acid | 9 ± 0 | 129,453 ± 6377 | 0.00 | 1.44 | 3.59 | Up |
| Phenolic acids | Glucosyloxybenzoic acid | 4,567,353 ± 3,606,822 | 19,471,000 ± 483,242 | 0.02 | 4.26 | 1.39 | Up |
| Isoflavones | 2′-Hydoxy,5-methoxy Genistein-4′,7-O-diglucoside | 119,761 ± 114,995 | 336,410 ± 31,108 | 0.07 | 2.81 | 1.18 | Up |
| Anthocyanins | Cyanidin | 112,889 ± 52,934 | 312,433 ± 11,394 | 0.02 | 2.77 | 1.14 | Up |
| Phenolamine | p-Coumaroylputrescine | 521,777 ± 394,617 | 1,562,433 ± 28,175 | 0.04 | 2.99 | 1.20 | Up |
| Lignans | Pinoresinol-acetylglucose | 371,567 ± 218,773 | 94,396 ± 16,341 | 0.16 | 2.50 | 1.12 | Down |
| Flavonoid carbonoside | 8-C-Hexosyl-chrysoeriol O-feruloylhexoside | 99,158 ± 24,712 | 39,812 ± 4298 | 0.04 | 4.00 | 1.07 | Down |
| Others | 3,5,7,4′–Tetrahydroxy-coumaronochromone | 1,925,333 ± 1,012,556 | 5,431,333 ± 79,192 | 0.03 | 2.82 | 1.15 | Up |
| Others | Androsin | 424,733 ± 270,074 | 91,641 ± 29,987 | 0.16 | 2.20 | 1.20 | Down |
Differentially accumulating compounds were identified using thresholds of VIP (variable importance in projection) ≥ 1 and fold change ≥ -1 (up-regulated) or ≤ 1 (down-regulated) in CK (transgenic Arabidopsis with empty vectors) compared to GlPS1(transgenic Arabidopsis with 35S:GlPS1). The mean and SD values for the peak area of each metabolite are listed. A two-tailed Student’s t test of the means was performed and p values which were over 0.05 (indicating non-significance) have been indicated in bold