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Effect of H. pylori products on cytokine production by resting and activated PBMCs from H. pylori-negative donors (n = 3). H. pylori products were urease (final concentration, 50 μg/ml), H. pylori French press lysate (50 μg of protein/ml), and intact bacteria (2.28 × 108 bacteria/ml) in vitro for 24 h with or without PHA plus PMA. Cytokine concentrations in culture supernatants were analyzed in duplicate by ELISA. Values are means ± standard errors.
