FIG. 2.

Effects of H. pylori factors on cytokine production by PBMCs. Cultures were performed as in Fig. 1 with various concentrations of H. pylori urease (12.5, 25, 50, and 100 μg/ml), French press lysate, and intact bacteria (equivalent to protein concentrations of 3.125, 6.25, 12.5, 25, 50, 100, and 200 μg of protein/ml each) in the absence (A) and presence (B) of PHA plus PMA. Values are means ± standard errors of 4 to 12 experiments. A one- or two-tailed Dunnett's test was used as appropriate. # and ##, significant increase (#, P < 0.05; ##, P < 0.01). ∗ and ∗∗, significant decrease (∗, P < 0.05; ∗∗, P < 0.01). The top row of symbols refers to urease values, the middle row refers to lysate, and the bottom row refers to intact bacteria.