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. 2022 Dec 21;8(51):eabq2202. doi: 10.1126/sciadv.abq2202

Fig. 7. Effect of crowders on the CD spectrum of Rec1-resilin.

Fig. 7.

(A) CD spectrum of pure Rec1-resilin and pure crowding agents. CD spectra of Rec1-resilin as a function of different crowding agents at different concentrations: (B) GLU, (C) GSH, (D) PEG3, (E) FIC70, and (F) DEX70. High concentrations of crowding agents (10% GSH and 40% others) resulted in high tension (HT) signal greater than 800 V (i.e., the noise becomes disproportionate to the signal) in the middle-UV range (<240 nm) for GSH and in the far-UV range (<200 nm) for GLU, PEG3, FIC70, and DEX70.