TABLE 1.
Bacterial plasmids used in this study
| Plasmid | Comment | Source or reference |
|---|---|---|
| pBK-CMV | Excised E. coli phagemid | Stratagene |
| pBK-CMV/BB | Excised S. aureus BB phagemid library | This study |
| pJM10 | pBK-CMV containing partial sst operon sequences | This study |
| pSPT18 | E. coli vector for making digoxigenin-labeled RNA probes with SP6 and T7 RNA polymerases | Boehringer Mannheim |
| pSPT18/sstD | pSPT18 with 0.96-kb SmaI/PstI fragment containing sstD | This study |
| pET30a | E. coli expression vector | Novagen |
| pET30a/sstD | pET30a containing sstD coding sequences | This study |
| pS10 | pMK4 E. coli-S. aureus shuttle vector with S. aureus S10 ribosomal gene promoter and T2 transcriptional terminator from E. coli | This study |
| pS1038 | pS10 with 0.96-kb SmaI/PstI fragment containing sstD | This study |