TABLE 2.

Effect of chemotherapy in ICAM-1 knockout mice

Mice and treatment	Liver parasite burden (LDU)		% Killing
	Day 0	Day +7a
Treatment started 2 wk after infectionb
C57BL/6 mice
No treatment	902 ± 125	1,097 ± 92	0
Sb (500 mg/kg, once)		132 ± 39**	85
AmB		73 ± 24**	92
ICAM-1-deficient mice
No treatment	1,507 ± 159	1,748 ± 100	0
Sb (500 mg/kg, once)		1,466 ± 129	3
Sb (500 mg/kg, three times)		1,386 ± 156	8
AmB		239 ± 64**	84
ICAM-1-deficient mice, treatment started 4 or 8 wk after infectionc
Wk 4
No treatment	2,254 ± 213	2,398 ± 191	0
Sb (500 mg/kg, once)		712 ± 102**	68
Wk 8
No treatment	1,326 ± 152	1,180 ± 156	11
Sb (500 mg/kg, once)		252 ± 51	81

^a**, P < 0.05 versus day 0 LDU value. 

^bTwo weeks after L. donovani challenge (day 0), mice received no treatment, a single injection of 500 mg of Sb/kg, three alternate-day injections of 500 mg of Sb/kg (ICAM-1-deficient mice), or three alternate-day injections of 5 mg of AmB/kg. Results are means ± standard errors of the means from three experiments for 9 to 11 mice at each time point except for ICAM-1-deficient mice treated three times with Sb (two experiments, n = 6 mice). 

^cTreatment with a single injection of Sb (500 mg/kg) was delayed until either 4 or 8 weeks after L. donovani challenge. Results in 4-week-infected mice are from two experiments (n = 6 to 7 mice); results in 8-week-infected mice are from one experiment (n = 3 mice).