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. 2022 Nov 17;135(19):2326–2337. doi: 10.1097/CM9.0000000000002154

Figure 2.

Figure 2

MiR-485-3p regulated PC cell migration and Invasion. (A, B) miR-485-3p was overexpressed and silenced In BxPC-3 and PANC-1 cells. (C, D) Transwell migration and Invasion assays in BxPC-3 and PANC-1 cells with overexpression or silencing of miR-485-3p (bar: 100 μm). The migrated or invaded cells were imaged at 200 × magnification in five random fields and counted using ImageJ software. (E) MiR-485-3p was overexpressed and silenced in PSCs. (F) FN1, COLIA1, and FAP levels in cell supernatant of PSCs transfected with miR-485-3p mimic or Inh-miR-485-3p were assessed by ELISA. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001,∗∗∗∗P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells.