Figure 8. Temporal relationship between connected Purkinje cells (PCs) and putative PC-MLIs.

(a and b) Average cross-correlograms calculated from significantly modulated pairs of MLIs and eyelid PCs. Background activity of putative PC-MLI is triggered off PC simple spikes (vertical red line), for PC-MLI and PC pairs recorded on the same tetrode (a) and from the computer simulation (b). Decrease in MLI activity after a PC simple spike indicates an inverse relationship between the activity of the two cell types. (c and d) Average PC pause-triggered (c) and burst-triggered (d) cross-correlograms of putative PC-MLI background activity. Plots show average PC-MLI activity across significant pairs recorded on the same tetrode. Pauses in PC simple spikes resulted in an increase in mean PC-MLI activity, while bursts of PC simple spikes resulted in a decrease in mean PC-MLI activity. MLIs, molecular layer interneurons.

Figure 8—figure supplement 1. Relationship between baseline activity of PC-MLIs and PCs simultaneously recorded from the same tetrode.

(a and b) Two examples of spike-triggered averages of MLI background activity triggered off PC simple spikes (vertical red line), recorded on the same tetrode. A total of six in vivo MLI/PC pairs showed a significant decrease in MLI activity after a PC spike. The post-spike decrease in MLI activity was abolished by shuffling the data. (c and d) Representative pause-triggered (c) and burst-triggered (d) cross-correlograms between background activity of putative PC-MLI (black) and eyelid PC simple spikes (green) recorded on the same tetrode for three different cell pairs. In all cases, pauses in PC simple spikes were associated with an increase in PC-MLI activity, while bursts of PC simple spikes were associated with a decrease in PC-MLI activity. MLI, molecular layer interneurons; PC, Purkinje cell.