Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Rnaseh2bf/f | PMID:2802351 | RRID:MGI:5911393 | Dr. Axel Roers (University of Technology Dresden) |
| Genetic reagent (M. musculus) | Setx-/- | PMID:23593030 | RRID:MGI:5697060 | Dr. Martin F Lavin (Queensland Institute of Medical Research) |
| Genetic reagent (M. musculus) | Cd19cre | PMID:9092650 | RRID:MGI:5614310 | Dr. Klaus Rajewsky (University of Cologne) |
| Genetic reagent (M. musculus) | Aicda-/- | PMID:11007474 | RRID:MGI:2156156 | |
| Recombinant DNA reagent | Migr1-RNaseH2B-FLAG | This paper | N-terminally FLAG tagged mouse Rnaseh2b |
|
| Antibody | Anti-S9.6 (mouse monoclonal) | Chedin lab | DRIP (1:500) Dot blot (1:1000) |
|
| Antibody | IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 680 (goat anti-mouse polyclonal) |
Thermo Fisher | Cat# A21057 | Dot blot (1:5000) |
| Antibody | APC CD19 (rat anti-mouse monoclonal) | BD Pharmingen | Cat# 561738 | Flow cytometry (1:100) |
| Antibody | PE-Cy7-CD43 (rat anti-mouse monoclonal) | BD Pharmingen | Cat# 562866 | Flow cytometry (1:20) |
| Antibody | PE -IgM (rat anti-mouse monoclonal) |
BD Pharmingen | Cat# 562033 | Flow cytometry (1:100) |
| Antibody | BV421-CD11b (rat anti-mouse monoclonal) | BD Pharmingen | Cat# 562605 | Flow cytometry (1:100) |
| Antibody | PerCP- CD45R/B220 (rat anti-mouse monoclonal) |
BD Pharmingen | Cat# 553093 | Flow cytometry (1:100) |
| Antibody | Anti-AID (mouse monoclonal) |
Thermo Fisher | Cat# 39-2500 | CHIP (1:200) WB (1:500) |
| Antibody | Anti-KU70/KU80 (mouse monoclonal) |
Invitrogen | Cat# MA1-21818 | CHIP (1:100) |
| Antibody | Anti-Rad52 (rabbit polyclonal) |
ABclonal | Cat# A3077 | CHIP (1:500) |
| Antibody | Anti-RNA polymerase II (mouse monoclonal) | Abcam | Cat# AB5408 | CHIP (1:500) |
| Antibody | IgG- Isotype Control (mouse polyclonal) | Abcam | Cat# AB37355 | CHIP (1:500) |
| Antibody | Anti-CD180 (rat anti-mouse monoclonal) | BD Pharmingen | Cat# 552128 | CSR (1:2000) |
| Peptide, recombinant protein | Recombinant murine IL-4 | PeproTech | Cat# 214-14 | CSR (1:2000) |
| Peptide, recombinant protein | Lipopolysaccharides | MilliporeSigma | Cat# L2630 | CSR (1:2000) |
| Commercial assay or kit | Beckman Coulter AMPURE XP | Beckman Coulter | Cat# A63881 | Size selection |
| Commercial assay or kit | SPHERO AccuCount Blank Particles | Spherotech | Cat# ACBP-50-10 | Flow cytometry |