FIG. 3.

Differential stimulatory capacity of OVA(257-264)/K^b and OVA(265-277)/I-A^b complexes after DC processing of Crl-OVA-expressing bacteria. DC were coincubated for 2 h with bacteria expressing Crl-OVA, as indicated on the x axis (see Fig. 1 legend). DC were then washed and treated with gentamicin, and incubations continued for an additional 22 h. After this 24-h period, either OVA(265-277)/I-A^b-specific OT4H T-hybridoma cells (A) or OVA(257-264)/K^b-specific CD8OVA T-hybridoma cells (B) were added for 24 h to quantitate OVA peptide presentation. Data are presented as the means of triplicate samples ± 1 standard deviation. Similar results were obtained in at least three independent experiments.