TABLE 1.
S. aureus strains used in this study
| Strain | Relevant genotypea | Relevant phenotype or propertiesa | Reference |
|---|---|---|---|
| 8325-4 | fnbA+ fnbB+ | FnBPA+ FnBPB+; NCTC 8325, cured of prophages, plasmid free | 25 |
| DU5883 | fnbA::TcrfnbB::Emr | FnBPA− FnBPB−; isogenic mutant of 8325-4 | 10 |
| DU5883(pFnBPA4) | Apr CmrfnbpA+ | FnBPA+ FnBPB−; DU5883 with multicopy shuttle plasmid carrying wild-type fnbpA | 10 |
| DU5875 | spa::Tcr | Spa−; isogenic mutant of 8325-4 | 23 |
a
fnbpA and fnbpB refer to the structural genes encoding the two staphylococcal FnBPs, A and B, and Spa refers to staphylococcal protein A.