TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. dysenteriae | ||
| SDU378 | Serotype 1 clinical isolate | A. Hartman |
| SDU380 | tonB | This study |
| SDU400 | shuA::mini-Tn10cam | This study |
| SDU402 | entB::Tn5, shuA::mini-Tn10cam | This study |
| E. coli | ||
| AB1515 | E. coli K-12 | C. F. Earhart |
| AB1515.24 | entB::Tn5 | 46 |
| CAG5053 | Hfr; KL208zbc-280::Tn10 | 45 |
| CAG5053entB::Tn5 | entB::Tn5 | This study |
| Plasmids | ||
| pCP410 | E. coli entEB(G)A in pACYC184 | 32 |
| pGTXN3 | Transcriptional fusion vector containing the promoterless mutant 3 gfp gene | 38 |
| pHTL116 | 2.6-kb EcoRV fragment containing shuA cloned into pWKS30 | 50 |
| pHEB1 | 6.6-kb EcoRI fragment of pCP410 cloned into pHTL116 | This study |
| pLR9 | 39-bp dnaY promoter (33) cloned upstream of gfp in pGTXN3 | L. Runyen-Janecky |
| pSc912 | shuA::mini-Tn10cam | 24 |
| pSR2 | 0.8-kb shuA promoter cloned upstream of gfp in pGTXN3 | This study |
| pYUK1 | E. coli tonB in pWSK129Cm | Y. Gleason |