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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Pain. 2021 Dec 15;163(8):1636–1647. doi: 10.1097/j.pain.0000000000002556

Figure 6.

Figure 6.

Ganglionic application of P2R inhibitors reduces the number of dorsal root ganglia neurons that respond to brush and heat stimulation at the hind paw. (A) Representative images show L4 DRG neuronal fluorescence in response to brush stimulation at the hind paw of Pirt-GCaMP6s mice before and 10 minutes after ganglionic application of A438079 (100 μM). (B–D) Quantification of neurons in each subpopulation that responded to brush stimulation before and after A317491 (100 μM, B), A438079 (100 μM, C), or probenecid (1 mM, D) treatment (n = 5 mice per group). (E) Representative images show L4 DRG neuronal fluorescence in response to 41°C heat stimulation at the hind paw of Pirt-GCaMP6s mice before and 10 minutes after ganglionic application of A317491 (100 μM). (F–H) Quantification of neurons in each subpopulation that responded to heat stimulation before and after A317491 (100 μM, F), A438079 (100 μM, G), or probenecid (1 mM, H) treatment (n = 5 mice per group). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs predrug (paired t test). DRG, dorsal root ganglia; GCaMP, genetically encoded calcium indicator; P2R, P2-purinergic receptor.