TABLE 1.
Bacterial strains and plasmids used in these studies
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strain | ||
| C. jejuni | ||
| M129 | Wild type; isolated from individual presenting frank dysentery | 18 |
| M129N | Spontaneous nalidixic acid-resistant strain, derivative of M129 | This study |
| JD900 | M129N derivative, katA::kan, Kanr Nalr | This study |
| JD901 | JD900 derivative, JD900::pJD107, Kanr Cmr Nalr | This study |
| E. coli | ||
| DH5α | F− φ80d lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1, Nalr | Bethesda Research Laboratories |
| UM255 | pro leu rpsL hsdM hsdR endI lacY recA katG2 katE12::Tn10, Tetr | 27 |
| S17-1 | thi thr leu tonA lacY supE recA::RP4-Z-Tc::(Mu kanr::smr) Smr | 20 |
| Plasmid | ||
| pUOA20 | E. coli-C. jejuni shuttle vector; source of cat gene (Cmr) | 36 |
| pRY107 | E. coli-C. jejuni shuttle vector; RP4 oriT aphA-3 (Kanr) Campylobacter ori | 36 |
| pRY107S | pRY107 derivative lacking a 1.8-kb HindIII fragment of the Campylobacter ori, Kanr | This study |
| pJD100 | pRY107 derivative containing C. jejuni katA gene at BamHI site | This study |
| pJD107 | pJD100 derivative containing the pUOA20 cat gene at the XbaI site, Cmr | This study |
| pJD108 | pRY107S derivative containing the 1.1-kb internal katA PCR product | This study |