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. 2022 Dec 8;13:1074867. doi: 10.3389/fimmu.2022.1074867

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Copyright © 2022 Wu, Wang, Ren, Li, Huang, Lin and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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As the experiment progressed, the NLRP3 inflammasome was activated and IL-1β expression increased in synovial tissue. (A) Western blot was conducted to evaluate the protein level of NLRP3 and GAPDH in synovial tissue (n=3). (B) Box plot showing the densitometry analysis of NLRP3 normalized by GAPDH. (C) The expression level of ASC in synovial tissue. (D) The expression level of Caspase-1 in synovial tissue. (E) The expression level of IL-1β in synovial tissue. Data are expressed as mean ± SEM for 8 quails in each group. *P < 0.05, **P < 0.01 and ***P < 0.001 versus Con group.