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. 2022 Dec 8;13:1074867. doi: 10.3389/fimmu.2022.1074867

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Copyright © 2022 Wu, Wang, Ren, Li, Huang, Lin and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Extraction and identification of FLS and its effect on cellular ROS and NLRP3 inflammasome after uric acid treatment. (A) Morphology of the first generation FLS. (B) Morphology of the third-generation FLS. (C) Vimentin identifies FLS as a characteristic labeling protein. (D) Detection of ROS expression levels in FLS after adding uric acid by flow cytometry. (E) The mean fluorescence intensity of FLS in each group. (F) Western blot was conducted to evaluate the protein level of NLRP3 and GAPDH in cell lysates. (G) Box plot showing the densitometry analysis of NLRP3 normalized by GAPDH. (H) The expression level of ASC in cell supernatant. (I) The expression level of Caspase-1 in cell supernatant. (J) The expression level of IL-1β in cell supernatant. *P < 0.05, **P < 0.01 and ***P < 0.001 versus Con group.