Figure 1.
Assembly and characterization of M2-nanodiscs
(A) The schematic diagram for M2-nanodisc assembly.
(B) The assembled nanodiscs were purified on Superdex 200 column by SEC.
(C) The peak fractions of SEC visualized on SDS-PAGE.
(D) DLS analysis for M2-nanodiscs and empty nanodiscs indicates a uniform distribution of M2-nanodiscs, with an average diameter of 10.29 nm, slightly larger than that of empty nanodiscs (8.964 nm).
(E) TEM data for empty nanodiscs (left) and M2-nanodiscs (right); images (scale bar: 100 nm or 20 nm) are shown on the top and 2D classifications (scale bar: 9 nm) at the bottom.