Figure 2.

The confirmation of tetramer formation of M2 inserted nanodiscs

(A) SDS-PAGE (left) and western blotting (right) stained with anti-M2 antibody (14C2) for purified nanodisc samples crosslinked with M2-nanodiscs by BS3. Lanes M1 and M, molecular mass markers; lane 1, M2(1-46); line 2, M2-nanodisc +1 mM BS³; line 3, MSPDH5; line4, MSPDH5 + 1 mM BS³.

(B and C) ESI mass spectra of crosslinked M2-nanodiscs with N terminal cross-linking analysis (B) and disulfide bond analysis (C).

(D) The schematic graph for a tetrameric M2 model based on the NMR structure (PDB: 2RLF).