REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Influenza A m2 Antibody (14C2)	Santa Cruz Biotechnology	Cat# sc-32238; RRID: AB_627808
His-Tag (D3I1O) XP® Rabbit mAb	Cell Signaling Technology (CST)	Cat# 12698S
Anti-mouse IgG, HRP-linked antibody	CST	Cat# 7076S
Anti-rabbit IgG, HRP-linked antibody	CST	Cat# 7074S
Anti-M13 (HRP)	HuaBio	Cat# EM1902-18
Bacterial and virus strains
SHuffle®T7CompetentE. coli	New England Biolabs (NEB)	Cat# C3026J
M13KO7 Helper Phage	New England Biolabs (NEB)	Cat# N0315S
ER2738	CRGEN	Cat# PB0421
Chemicals, peptides, and recombinant proteins
1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (16:0-18:1 PC (POPC))	Avanti Polar Lipids	Cat# 850457P
M2e	GL Biochem (Shanghai) Ltd	N/A
M2(1-46)	GL Biochem (Shanghai) Ltd	N/A
M2(1-60)	GL Biochem (Shanghai) Ltd	N/A
Molecular weight ladder (M)	BBI life sciences corporation	Cat# C600201
Molecular weight ladder (M1)	Biosharp	Cat# BL712A
Freunds Adjuvant, complete	BIOFROXX	Cat# 2203ML010
Freund’s Adjuvant incomplete	BIOFROXX	Cat# 1643ML010
sfiI	NEB	Cat# R0123L
T4 DNA ligase	NEB	Cat# M0202T
pComb3XSS	Andris-Widhopf et al.,48	Cat# 63890 (Addgene)
pcold II	Sangon	Cat# B540188-0001
3,3′-Dioctadecyloxacarbocyanine perchlorate (Dio)	MCE	Cat# 34215-57-1
Sulfo-Cyanine5 Succinimidyl Ester(Cy5-SE)	MCE	Cat# 146368-14-1
Deposited data
NMR structure	Schnell et al.,46	PDB: 2RLF (https://www.rcsb.org)
Oligonucleotides
5′-CGTGGCCCAGGCGGCCATGGCC SMACGGSTTGAACAAACACC-3′	This study	N/A
5′-GCTGGCCGGCCTGGCCWTTCA CAGTCASARKGGTSCC-3′	This study	N/A
Software and algorithms
EMAN2	Tang et al.,68	https://cryoem.bcm.edu/cryoem/downloads/view_eman2_versions
relion3.1.3	Scheres et al.,69	https://relion.readthedocs.io/en/release-3.1/index.html
ImageJ	National Institute of Health	RRID:SCR_003070; https://imagej.net/
Graphpad Prism 7.0	GraphPad Software	https://www.graphpad.com/