REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CR3022	Genscript (https://www.genscript.com)	N/A
P2B-2F6	Dr Nicole Doria-Rose, VRC, USA	N/A
084-7D	This paper	N/A
AIRU946-E4	This paper	N/A
AIRU946-A6	This paper	N/A
anti-IgG APC (clone QA19A42)	Biolegend	Cat#366905; RRID:AB_2888847
Palivizumab	Medimmune	Synagis; RRID: AB_2459638
Bacterial and virus strains
SARS-CoV-2 pseudoviruses for ancestral, Beta, Delta, Omicron BA.1, Omicron BA.2, Omicron BA.4	Wibmer et al.1; Richardson et al.,16 this paper	N/A
Biological samples
Convalescent hospitalized blood samples	Groote Schuur Hospital	https://www.gsh.co.za
Convalescent hospitalized blood samples	Steve Biko Academic Hospital	https://www.sbah.org.za
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 original variant spike proteins	Original: Dr Jason McKellan	N/A
Critical commercial assays
PEI-MAX 40,000	Polysciences	Cat # 24765-1
QUANTI-Luc luciferase	Invivogen	Cat# rep-qlc2
Luciferase	Promega	Cat# PRE263B-C
Experimental models: Cell lines
Human Embryonic Kidney (HEK) 293F	Dr Nicole Doria-Rose, VRC, USA	N/A
HEK293T/ACE2.MF	Dr Michael Farzan, Scripps, USA	N/A
Jurkat-Lucia™ NFAT-CD16 cells	Invivogen	Cat # jktl-nfat-cd16
Human Embryonic Kidney (HEK) 293T cells	Dr George Shaw, UPenn,USA	N/A
Recombinant DNA
Spike Hexapro plasmid	Original: Dr Jason McKellan Beta: Moyo-Gwete et al.36	N/A
SARS-CoV-2 ancestral variant spike (D614G) plasmid	Wibmer et al.1	N/A
Beta spike (L18F, D80A, D215G, K417N, E484K, N501Y, D614G, A701V, 242–244 del) plasmid	Wibmer et al.1	N/A
Delta spike (T19R, R158G L452R, T478K, D614G, P681R, D950N, 156–157 del) plasmid	Richardson et al.16	N/A
Omicron BA.1 plasmid (A67V, Δ69–70, T95I, G142D, Δ143-145, Δ211, L212I, 214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)	Richardson et al.16	N/A
Omicron BA.2 plasmid (T19I, L24S, 25-27del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S,K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K)	Richardson et al.16	N/A
Omicron BA.4 plasmid (T19I, L24S, Δ25-27, Δ69-70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K).	This paper	N/A
Firefly luciferase encoding lentivirus backbone plasmid	Dr Michael Farzan, Scripps	N/A
Software and algorithms
FACSDiva 9	BD Biosciences	https://www.bdbiosciences.com
FlowJo 10	FlowJo, LLC	https://www.flowjo.com
Graphpad Prism 9	Graphpad	https://www.graphpad.com
Biorender	Biorender	https://www.biorender.com