Extended Data Fig. 6. Suppression of BAT activation and vWAT browning by VEGF blockade in SARS-CoV-2-infected Syrian hamsters.
(a) BAT and vWAT weights in NI- hamsters and NIIgG- or anti-VEGF-treated day-8-post SC-infected hamsters (n = 5 hamsters per group). (b) Histological and immunohistochemical analyses of BAT and vWAT of NI- hamsters and NIIgG- or anti-VEGF-treated day-8-post SC-infected hamsters by staining with H&E, Perilipin (PERI), UCP1 and COX4. Tissue sections were counterstained with DAPI (blue). Quantification of UCP1 and COX4 positive signals in BAT and vWAT (n = 8 random fields per group). (c) mRNA levels of browning markers of Ucp1, Cox4, Dio2, Tbx1, Tnfrsf9, and Pdgfra in BAT and vWAT of NI- hamsters and NIIgG- or anti-VEGF-treated day-8-post SC-infected hamsters were quantified by qPCR (n = 6 samples per group). (d) mRNA levels of Car9, Hif1a and Vegf in BAT and vWAT of NI- hamsters and NIIgG- or anti-VEGF-treated day-8-post SC-infected hamsters were quantified by qPCR (n = 6 samples per group). Data are presented as means ± s.e.m. Statistical analysis was performed using two-sided unpaired t-tests. NS = not significant. NI = non-infected. SC = SARS-CoV-2. Scale bar = 50 μm.