Extended Data Fig. 1. SARS-CoV-2 instigates adipose browning.
(a) Representative images of BAT and quantification of brown adipose depot weights of NI- or day-6-post SC-infected mice (n = 6 samples per group). (b) Daily food intake of NI- and SC-infected mice (n = 6 mice per group). (c) mRNA levels of browning markers of Cox7a, Cox8b, Cidea, and Prdm16 in BAT and sWAT of NI- or day-6-post SC-infected mice were quantified by qPCR (n = 8 samples per group). (d) Body weight of mice at various infected time points (n = 4 mice per group). (e) mRNA levels of browning markers of Ucp1, Cox4 in BAT and sWAT of mice at various infected time points were quantified by qPCR (n = 4 samples per group). (f) Histological and immunohistochemical analyses of BAT and sWAT of mice at various infected time points by staining with H&E, perilipin (PERI), UCP1 and COX4. Immunohistological sections were counterstained by DAPI (blue). UCP1 and COX4 positive signals of BAT and sWAT were quantified (n = 8 random fields per group). Data are presented as means ± s.e.m. Statistical analysis was performed using two-sided unpaired t-tests. NS = not significant. SC = SARS-CoV-2. Scale bar = 50 μm.