Extended Data Fig. 4. Maximum likelihood phylogenetic trees of the 16S rRNA genes from single cells obtained from the Gulf of Maine in 2019, complemented with cell respiration and diameter measurements.

A. SAGs from April 2, 2019. B. SAGs from July 9, 2019. In both trees, cultured isolates are included as phylogenetic references and are indicated by red font colour. The trees were rooted in the Archaeal branch, when present, and at the midpoint when no archaea are included.