Fig. 5. Viral-mediated over-expression of RBFOX2-Δ6 and RBFOX2 WT in the liver.
a, Cartoon depicting the AAV backbones used to over-express RBFOX2-Δ6 (with a truncated RNA-binding motif RRM) or control green fluorescent protein (GFP) in the liver (top) and representative western blot showing expression levels in the liver of male mice (n = 3). b, RT–qPCR expression analysis of codon-optimized RBFOX2-Δ6 in the liver (n = 6–9). c, Quantification of PSI for Numb (exon 3), Osbpl9 (exon 6), Scarb1 (exon 12) and Sec31a (exon 23) (n = 9–10). d, Cartoon depicting the adenoviral backbones used to over-express RBFOX2 wild type or control GFP (top) and representative western blot showing expression levels in hepatocytes (n = 3). e, Capillary electrophoresis and quantification of PSI for Numb (exon 3), Osbpl9 (exon 6), Scarb1 (exon 12) and Sec31a (exon 23) after RBFOX2 over-expression in hepatocytes (n = 6). f, LC–MS lipidomic analysis showing total levels of free cholesterol, cholesteryl ester, sphingomyelin and ceramide normalized to liver tissue mass and PUFA/non-PUFA TG ratio in male mice fed a HFr diet after transduction with pAd-RBFOX2 or pAd-GFP control (n = 8–9). g, Cholesterol levels quantified in the bile of mice fed a HFr diet after transduction with pAd-RBFOX2 or pAd-GFP control (n = 8–9). h, Plasma lipid analysis showing total cholesterol, HDL cholesterol and TGs in mice fed a HFr diet after transduction with pAd-RBFOX2 or pAd-GFP control (n = 8–9). Results are represented as mean ± s.e.m. Statistical significance was determined by two-tailed unpaired t-test of biologically independent samples.