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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Arthritis Rheumatol. 2022 Oct 31;74(12):2003–2014. doi: 10.1002/art.42286

Figure 2.

Figure 2.

Single-cell RNA-sequencing analysis of macrophage subpopulations, dendritic cells, proliferating Mφ from 5 lung samples in 3’ v3 chemistry, 1 healthy control and 4 SSc-ILD lung samples. (A) Visualization of clustering by UMAP per cell type. (B) Visualization of clustering by UMAP per health status. (C) Visualization of gene expression by FeaturePlot of macrophage subpopulations gene markers, dendritic cells, proliferating Mφ and other genes expression during analysis of only macrophages, dendritic cells, proliferating Mφ. (D) Visualization of gene expression by DotPlot of macrophages, dendritic cells, proliferating Mφ from 1 healthy control and 4 SSc-ILD lung samples in scRNA-seq 3’ v3 Chemistry. DotPlot shows upregulation of SPP1, MERTK, LGMN genes in SSc-ILD SPP1 Mφ and proliferating Mφ. MMP9 and PLA2G7 genes markers are upregulated in SSc-ILD SPP1 Mφ compared to all other macrophages.