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. 2022 Oct 18;74(12):2024–2031. doi: 10.1002/art.42283

Figure 1.

Figure 1

CD14+ macrophages, plasmacytoid dendritic cells (pDCs), and CD8+ T cells were increased in childhood‐onset systemic lupus erythematosus (cSLE) compared to juvenile dermatomyositis (JDM) lesional skin. A, Multiplexed images demonstrating staining for cellular markers in JDM and cSLE skin samples, represented by different colors in panels 1–5. Bars = 100 μm. B and C, Analysis by t‐distributed stochastic neighbor embedding (t‐SNE) dimensionality reduction demonstrating overlay of identified JDM and cSLE cell clusters (B) and individual t‐SNE plots by disease (C). D, Phenograph clustergram and heatmap showing marker expression by cell cluster. E, Quantification of immune cell types per disease based on marker expression. Bars show the mean ± SEM. * = P < 0.05. F, Representative images demonstrating higher quantities of CD14+ macrophages, pDCs, and CD8+ T cells in cSLE compared to JDM. Magnification is the same as in A. BDCA2 = blood dendritic cell antigen 2; mDCs = myeloid DCs.