Figure 7:

Tissue sensitivity to TH in tail and hindlimbs among MCT8 genotypes. Premetamorphic F3 tadpoles (NF 54) were treated for 24 hrs with 0, 0.5, 2, and 5 nM T3 (tri-iodothyronine) added to their rearing water, and then their tails and hindlimbs were harvested and processed for quantitative PCR. The RNA expression of the TH response genes, (A,C) Krüppel-like factor 9 (klf9) and (B, D) TH receptor beta (thrb) in tail (A,B) and hindlimbs (B,C) among MCT8 genotypes. Means and standard error for each genotype are shown, n = 10 for all genotypes and T3 treatments. WT = wild-type (LLss), MCT8KO = homozygous knockout (llss). Letters indicate significant differences among bars determined for each genotype based on non-parametric Kruskal-Wallis test followed by pairwise comparisons using Wilcoxon rank sum exact test (A, B and D) and two-way ANOVA followed by pairwise comparisons using Tukey’s post hoc test (C). p < 0.05.