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Gene-specific primers for TH response gene expression analysis
| Gene (L- and/or S-chromosome) | Primer sequence (5’ → 3’) |
|---|---|
| thrb (both L and S) | F: AAGCCTTCAGCCAGTTTACA R: GAGCGACATGATCTCCATACAA |
| rpl8 (both L and S) | F: AGAAGGTCATCTCATCTGCTAAC R: GGATAGGTTTGTCAATACGACCA |
|
klf9 (both L and S) (F primer has 1 mismatch in S chromosome) |
F: TACTGGGTGTGGCAAAGTTTAT R: CTCTTCTCACAGAGTGGACATC |
| tshb (L only) | F: GTTCAGGATTCTGCATGACAAGG R: ACAGTCAGTGTAGCCAGTGTTA |
